‹ zurück Sie sind hier:  Startseite » Buchhändler » MARZIES.de Buch- und Medienhan… » cDNA Libraries Methods and Applications

Linksbuch Neu neu Rechtsbuch

Lu, Chaofu (Herausgeber); Browse, John (Herausgeber); Wallis, James G. (Herausgeber)

cDNA Libraries Methods and Applications (Gebundene Ausgabe)


Reihe: Methods in Molecular Biology 729

Springer-Verlag GmbH, August 2011


275 S. - Sprache: Englisch - 45 schwarz-weiße und 2 farbige Abbildungen, 5 schwarz-weiße Tabellen - 261x189x20 mm Book

ISBN: 1617790648 EAN: 9781617790645

The numerous vital applications of complementary DNA (cDNA) technology have changed dramatically as the technology has advanced over recent years.  In cDNA Libraries: Methods and Protocols, expert researchers provide current techniques that reflect the latest advances in the construction and application of cDNA libraries.  The first half of the volume covers improved approaches to some of the most basic elements of creating cDNA libraries, while the second half casts a much wider net and includes visionary applications of cDNA technology which were either unforeseen or technically impractical until recently.  Written in the highly successful Methods in Molecular Biology(TM) series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.  Authoritative and cutting-edge, cDNA Libraries: Methods and Protocols serves as an ideal guide to all scientists seeking to advance this important technology and provide answers to the enduring fundamental questions of biology.


Preis: 128,39 EUR
Versandkosten: 0,00 EUR
Gesamtpreis: 128,39 EUR
In den Warenkorb

Anbieter: MARZIES.de Buch- und Medienhan… (Impressum/Lieferzeit)
Katalog: HC/Medizin/Andere Fachgebiete

Angebotene Zahlungsarten: Vorauskasse
Transparente Bestellabwicklung - Verkäufer nutzt Order-Control - klicken für mehr Information
Transparente Bestellabwicklung -
Verkäufer nutzt Order-Control

 

Produktdetails

Captures the current state of this ever-developing and powerful technologyFeatures practical methodologies, ready for the labContains vital implementation advice from experts in the field

Inhaltsverzeichnis

Part I: Enhanced Approaches to Inherent Problems?1. Isolation of Fungal Infection Structures from Plant Tissue by Flow Cytometry for Cell-Specific Transcriptome Analysis??????????? Hiroyuki Takahara, Elmar Endl, and Richard O'Connell?2. Preparation of a High Quality cDNA Library from a Single-Cell Quantity of mRNA Using Chum-RNA??????????? Hiroshi Nojima and Takahiro Tougan?3. Construction of a Full-Length cDNA Library from Castor Endosperm for High-throughput Functional Screening??????????? Chaofu Lu, James G. Wallis, and John Browse?4. Full-Length Transcriptome Analysis Using a Bias-Free cDNA Library Prepared with the Vector-Capping Method??????????? Seishi Kato, Mio Oshikawa, and Kuniyo Ohtoko?5. Construction of Improved Yeast Two-Hybrid Libraries??????????? Richard H. Maier, Christina J. Maier, and Kamil ?nder?6. Normalization of Full-Length Enriched cDNA??????????? Ekaterina A. Bogdanova, Ekaterina V. Barsova, Irina A. Shagina, Alexander Scheglov, Veronika Anisimova, Laura L. Vagner, Sergey A. Lukyanov, and Dmitry A. Shagin?7. Bioinformatic Methods for Finding Differentially Expressed Genes in cDNA Libraries, Applied to the Identification of Tumour Vascular Targets??????????? John M.J. Herbert, Dov J. Stekel, Manuela Mura, Michail Sychev, and Roy Bicknell?Part II: Visionary Applications?8. Enzymatic Production of RNAi Libraries from cDNAs and High-Throughput Selection of Effective shRNA Expression Constructs??????????? Kohtaroh Sugao and Kenzo Hirose?9. Construction of Small RNA cDNA Libraries for High-Throughput Sequencing??????????? Cheng Lu and Vikas Shedge?10. Focusing Mutations Within Random Libraries to Distinct Areas: Protein Domain Library Generation by Overlap Extension (PDLGO)??????????? Andreas Gratz and Joachim Jose?11. Generation of Families of Construct Variants Using Golden Gate Shuffling??????????? Carola Engler and Sylvestre Marillonnet?12. Application of Full-Length cDNA Resources to Gain-of-Function Technology for Characterization of Plant Gene Function??????????? Youichi Kondou, Mieko Higuchi, Takanari Ichikawa, and Minami Matsui?13. Construction of Yeast Surface-Displayed cDNA Libraries??????????? Scott Bidlingmaier and Bin Liu?14. Identification of Protein/Target Molecule Interactions Using Yeast Surface-Displayed cDNA Libraries??????????? Scott Bidlingmaier and Bin Liu?15. SNP Discovery by Transcriptome Pyrosequencing??????????? W. Brad Barbazuk and Patrick S. Schnable?16. RNA-seq Analysis of Gene Expression and Alternative Splicing by Double-Random Priming Strategy??????????? Michael T. Lovci, Hai-Ri Li, Xiang-dong Fu, and Gene W. Yeo?17. Generation of a Large Catalog of Unique Transcripts for Whole-Genome Expression Analysis in Non-Model Species??????????? Diana Bellin, Alberto Ferrarini, and Massimo Delledonne


ISBN 1-617790-64-8, ISBN 1-61-779064-8, ISBN 1-61779-064-8, ISBN 1-617-79064-8, ISBN 1-6177-9064-8

ISBN 978-1-617790-64-5, ISBN 978-1-61-779064-5, ISBN 978-1-61779-064-5, ISBN 978-1-617-79064-5, ISBN 978-1-6177-9064-5

Buch empfehlen Buch empfehlen
Sparen Sie Versandkosten beim Kauf weiterer Artikel dieses Buchhändlers.

Aquatic Dermatology Biotic, Chemical and Physical Agents von Bonamonte, Domenico (Herausgeber); Angelini, Gianni (Herausgeber)
139,99 EUR

End-of-Life Care in Cardiovascular Disease von Goodlin, Sarah J. (Herausgeber); Rich, Michael W. (Herausgeber)
139,99 EUR

129,99 EUR

Kindling 6 von Corcoran, Michael E. (Herausgeber); Moshe, Solomon L. (Herausgeber)
319,93 EUR

CT Colonography Atlas For the Practicing Radiologist von Neri, Emanuele (Herausgeber); Faggioni, Lorenzo (Herausgeber); Bartolozzi, Carlo (Herausgeber)
159,99 EUR

Magnetic Resonance Imaging of the Knee von Niitsu, Mamoru; Hayashi, Daichi (Übersetzung)
159,99 EUR
Meine zuletzt angesehenen antiquarischen und neue Bücher
Meine letzten Buch Suchanfragen
    Noch keine Suchanfragen vorhanden.

© 2003 - 2018 by whSoft GmbH
Entdecken Sie außerdem: Antiquariatsportal · Achtung-Bücher.de · Booklink · Buchhai - Bücher Preisvergleich
Alle genannten Preise sind Bruttopreise und enthalten, soweit nicht anders vom Verkäufer angegeben, die gesetzliche Umsatzsteuer.