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Merten, Otto-Wilhelm (Herausgeber); Al-Rubeai, Mohamed (Herausgeber)

Viral Vectors for Gene Therapy Methods and Protocols (Gebundene Ausgabe)

Reihe: Methods in Molecular Biology 737

Springer-Verlag GmbH, Humana Press, November 2011

450 S. - Sprache: Englisch - 53 schwarz-weiße und 3 farbige Abbildungen, 31 schwarz-weiße Tabellen - 262x192x30 mm Book

ISBN: 161779094X EAN: 9781617790942

The huge potential for gene therapy to cure a wide range of diseases has led to high expectations and a great increase in research efforts in this area, particularly in the study of delivery via viral vectors, widely considered to be more efficient than DNA transfection.  In Viral Vectors for Gene Therapy: Methods and Protocols, experts in the field present a collection of their knowledge and experience featuring methodologies that involve virus production, transferring protocols, and evaluating the efficacy of gene products. While thoroughly covering the most popular viral vector systems of adenovirus, retrovirus, and adeno-associated virus, this detailed volume also explores less common viral vector systems such as baculovirus, herpes virus, and measles virus, the growing interest in which is creating a considerable demand for large scale manufacturing and purification procedures.  Written in the highly successful Methods in Molecular Biology(TM) series format, many chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and vital tips on troubleshooting and avoiding known pitfalls.  Comprehensive and practical, Viral Vectors for Gene Therapy: Methods and Protocols provides basic principles accessible to scientists from a wide variety of backgrounds for the development of gene therapy viral products that are safe and effective.

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Focuses on the many applications of viral vectors, widely considered more efficient than DNA transfectionContains practical laboratory protocols using a wide array of common and uncommon virusesFeatures expert tips and key implementation advice


1. Introduction to Viral Vectors??????????? James N. Warnock, Claire Daigre, and Mohamed Al-Rubeai?2. Introduction to Gene Therapy: A Clinical Aftermath??????????? Patrice P. Den?fle?3. Host Cells and Cell Banking??????????? Glyn N. Stacey and Otto-Wilhelm Merten?4. Overview of Current Scalable Methods for Purification of Viral Vectors??????????? Mar?a Mercedes Segura, Amine Kamen, and Alain Garnier?5. Methods to Construct Recombinant Adenovirus Vectors??????????? Miguel Chillon and Ramon Alemany?6. Manufacturing of Adenoviruses Vectors: Production and Purification of Helper Dependent Adenovirus??????????? Edwige Dormond and Amine A. Kamen?7. Manufacturing of Retroviruses??????????? Pedro E. Cruz, Teresa Rodrigues, Marlene Carmo, Dagmar Wirth, Ana Amaral, Paula M. Alves, and Ana S. Coroadinha?8. Lentiviral Vectors??????????? Marc Giry-Laterri?re, Els Verhoeyen, and Patrick Salmon?9. Adeno-Associated Viruses??????????? Mauro Mezzina and Otto-Wilhelm Merten?10. Manufacturing of Adeno-Associated Viruses, for Example: AAV2??????????? Haifeng Chen?11. Vector Characterization Methods for Quality Control Testing of Recombinant Adeno-Associated Viruses??????????? J. Fraser Wright and Olga Zelenaia?12. Baculoviruses Mediate Efficient Gene Expression in a Wide Range of Vertebrate Cells??????????? Kari J. Airenne, Kaisa-Emilia Makkonen, Anssi J. M?h?nen, and Seppo Yl?-Herttuala?13. Herpes Simplex Virus Type 1 (HSV-1)-Derived Recombinant and Amplicon Vectors??????????? Cornel Fraefel, Peggy Marconi, and Alberto L. Epstein?14. Manufacture of Measles Viruses??????????? Kirsten K. Langfield, Henry J. Walker, Linda C. Gregory, and Mark J. Federspiel?15. In Vivo Gene Delivery into hCD34+ Cells in a Humanized Mouse Model??????????? Cecilia Frecha, Floriane Fusil, Fran?ois-Lo?c Cosset, and Els Verhoeyen?16. In Vivo Evaluation of Gene Transfer into Mesenchymal Cells (in View of Cartilage Repair)??????????? Kolja Gelse and Holm Schneider?17. Ethical Consideration??????????? Michael Fuchs?18. Clinical Trials of GMP Products in the Gene Therapy Field??????????? Kathleen B. Bamford


From the reviews:"Intended for practitioners involved in the preclinical and clinical applications of gene therapy vectors. ... students, scientists, and physicians specializing in gene therapy will find this book extremely useful. ... Given the rapid advances in the field of gene therapy, this book is essential, providing useful information on the methodologies that involve virus production, transferring protocols, and evaluation of the efficacy of gene products and other important methodological concepts in gene therapy." (Omer Iqbal, Doody's Review Service, September, 2011)

ISBN 1-617790-94-X, ISBN 1-61-779094-X, ISBN 1-61779-094-X, ISBN 1-617-79094-X, ISBN 1-6177-9094-X

ISBN 978-1-617790-94-2, ISBN 978-1-61-779094-2, ISBN 978-1-61779-094-2, ISBN 978-1-617-79094-2, ISBN 978-1-6177-9094-2

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