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Popper, Zoë A. (Herausgeber)

The Plant Cell Wall Methods and Protocols (Gebundene Ausgabe)

Reihe: Methods in Molecular Biology 715

Springer-Verlag GmbH, Humana Press, April 2011

310 S. - Sprache: Englisch - 41 schwarz-weiße und 30 farbige Abbildungen, 15 schwarz-weiße Tabellen - 266x184x25 mm Book

ISBN: 1617790079 EAN: 9781617790072

The cell wall and its constituent polysaccharides and proteins control nearly all plant-based biological and biophysical processes. Understanding the cell wall is, therefore, not only fundamental to the plant sciences but is also pertinent to aspects of human and animal nutrition and health as well as plant-microbe and plant-animal interactions. In The Plant Cell Wall: Methods and Protocols, experts in the field describe detailed methods which are currently being applied to investigate the many aspects of the plant cell wall including its structure, biochemical composition, and metabolism.  The book delves into a range of techniques involving plant tissue culture, which can be applied to investigating cell wall structure and metabolism, methods directed towards structural analysis and occurrence of carbohydrates, the development and use of microscopy-based tools and techniques, procedures which measure the physical properties of the wall, and methods based on the application of molecular genetic approaches.  Written in the successful Methods in Molecular Biology(TM) series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.  Authoritative and easily accessible, The Plant Cell Wall: Methods and Protocols seeks to serve both professionals and novices with its well-honed methodologies in an effort to further our knowledge of this essential cellular feature.

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-Features many recently developed methods, becoming more widely used with the development of advanced instrumentation and technology-Contains detailed protocols to study plant cell wall structure, biochemical composition, and metabolism, to name a few areas-Includes expert tips and vital implementation advice


1. Plant Tissue Cultures??????????? Anna K?rk?nen, Arja Santanen, Kuninori Iwamoto, and Hiroo Fukuda?2. Computerized Molecular Modelling of Carbohydrates??????????? Alfred D. French and Glenn P. Johnson?3. Oligosaccharide Mass Profiling (OLIMP) of Cell Wall Polysaccharides by MALDI-TOF/MS??????????? Markus G?nl, Florian Kraemer, and Markus Pauly?4. High-Voltage Paper Electrophoresis (HVPE) of Cell-Wall Building Blocks and Their Metabolic Precursors??????????? Stephen C. Fry?5. Carbohydrate Gel Electrophoresis??????????? Florence Goubet, Paul Dupree, and Katja Salomon Johansen?6. Capillary Electrophoresis with Detection by Laser-Induced Fluorescence??????????? Andrew Mort and Xiangmei Wu?7. Monoclonal antibodies, Carbohydrate-Binding Modules, and the Detection of Polysaccharides in Plant Cell Walls??????????? C?cile Herv?, Susan E. Marcus, and J. Paul Knox?8. Screening and Characterization of Plant Cell Walls Using Carbohydrate Microarrays??????????? Iben S?rensen and William G.T. Willats?9. Electron Tomography and Immunogold Labelling as Tools to Analyze de novo Assembly of Plant Cell Walls??????????? Marisa S. Otegui?10. Analyzing Cellulose Biosynthesis with Confocal Microscopy??????????? Meera Nair and Seth DeBolt?11. Visual Mapping of Cell Wall Biosynthesis??????????? Yumiko Sakuragi, Morten H.H. N?rholm, and Henrik V. Scheller?12. Atomic Force Microscopy of Plant Cell Walls??????????? Andrew R. Kirby?13. Using Solid-State 13C NMR Spectroscopy to Study the Molecular Organization of Primary Plant Cell Walls??????????? Tracey J. Bootten, Philip J. Harris, Laurence D. Melton, and Roger H. Newman?14. Formation of Cellulose-Based Composites with?Hemicelluloses and Pectins Using?Gluconacetobacter Fermentation??????????? Deirdre Mikkelsen and Michael J. Gidley?15. Structural Proteins of the Primary Cell Wall: Extraction, Purification, and Analysis??????????? Derek T.A. Lamport, Li Tan, and Marcia J. Kieliszewski?16. New Insights into the Control of Cell Growth??????????? Claudia Blaukopf, Matth?us Z. Krol, and Georg J. Seifert?17. Extraction and Detection of Arabinogalactan Proteins??????????? Zo? A. Popper?18. Characterization of the Plant Cell Wall Proteome Using High Throughput Screens??????????? Sang-Jik Lee and Jocelyn K.C. Rose?19. Knocking Out the Wall: Protocols for Gene Targeting in Physcomitrella patens??????????? Alison W. Roberts, Christos S. Dimos, Michael J. Budziszek, Jr., Chessa A. Goss, and Virginia Lai?20. Measuring In vitro Extensibility of Growing Plant Cell Walls??????????? Daniel J. Cosgrove

ISBN 1-617790-07-9, ISBN 1-61-779007-9, ISBN 1-61779-007-9, ISBN 1-617-79007-9, ISBN 1-6177-9007-9

ISBN 978-1-617790-07-2, ISBN 978-1-61-779007-2, ISBN 978-1-61779-007-2, ISBN 978-1-617-79007-2, ISBN 978-1-6177-9007-2

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