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Rumbaugh, Kendra P. (Herausgeber)

Quorum Sensing Methods and Protocols (Gebundene Ausgabe)

Reihe: Methods in Molecular Biology 692

Springer-Verlag GmbH, Humana Press, Dezember 2010

315 S. - Sprache: Englisch - 79 schwarz-weiße und 79 farbige Abbildungen, 8 schwarz-weiße Tabellen - 263x187x30 mm

ISBN: 1607619709 EAN: 9781607619703

Since its early days in the 1990s, the Quorum Sensing (QS) field has grown from a few dozen laboratories, investigating the pathways, proteins, and chemicals that facilitate signaling in bacteria, to hundreds of groups that have integrated evolutionary biology, computer science, mathematics, engineering, and metagenomics to create an ever-expanding and dynamic field. In Quorum Sensing: Methods and Protocols, expert researchers provide an in-depth set of diverse protocols that span this broad area of study. Broken into three detailed sections, the volume covers the detection, isolation, and characterization of the QS signals made by both Gram- and Gram+ bacteria, determination of the function of QS signals in vivo, and the development of QS disruption strategies. Written in the highly successful Methods in Molecular Biology(TM) series format, chapters include brief introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and expert tips on troubleshooting and avoiding known experimental pitfalls. Comprehensive and cutting-edge, Quorum Sensing: Methods and Protocols serves as an invaluable collection of easily accessible techniques for scientists seeking to further our knowledge about bacterial communication and its relation to humanity.

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Features in-depth protocols on detecting, isolating, characterizing QS signals, function determination, and QS disruption strategies Contributions from expert researchers who have experienced the field from its beginnings to the current cutting-edge studies Includes key notes on avoiding known protocol pitfalls and troubleshooting potential problems


Foreword I by E. Peter Greenberg Foreword II by Paul Williams Part I: Detection, Isolation, and Characterization of Quorum Sensing Compounds 1. Bioassays of Quorum Sensing Compounds Using Agrobacterium tumefaciens and Chromobacterium violaceum Weihua Chu, Dhiraj A. Vattem, Vatsala Maitin, Mary B. Barnes, and Robert J.C. McLean 2. Detection of 2-alkyl-4-quinolones Using Biosensors Stephen P. Diggle, Matthew P. Fletcher, Miguel C?mara, and Paul Williams 3. FRET-Based Biosensors for the Detection and Quantification of AI-2 Class of Quorum Sensing Compounds Sathish Rajamani and Richard Sayre 4. Isolation of agr Quorum Sensing Autoinducers Naomi Balaban 5. Liquid Chromatography/Mass Spectrometry (LC/MS) for the Detection and Quantification of N-acyl-L-homoserine Lactones (AHLs) and 4-hydroxy-2-alkylquinolines (HAQs) Fran?ois L?pine and Eric D?ziel 6. Detection of Autoinducer (AI-2) like Activity in Food Samples Kirthiram K Sivakumar, Palmy R. Jesudhasan, and Suresh D. Pillai 7. Detection of Bacterial Signaling Molecules in Liquid or Gaseous Environments Peter Edmonson, Desmond Stubbs, and William Hunt 8. Determination of acyl Homoserine Lactone and Tetramic Acid Concentrations in Biological Samples Colin A. Lowery, Gunnar F. Kaufmann, and Kim D. Janda 9. Luminescent Reporters and Their Applications for the Characterization of Signals and Signal-Mimics that Alter LasR-Mediated Quorum Sensing Ali Alagely, Sathish Rajamani, and Max Teplitski Part II: Determining the Function of Autoinducers In vivo 10. Modulation of Mammalian Cell Processes by Bacterial Quorum Sensing Molecules Vladimir V. Kravchenko, Richard J. Ulevitch, and Gunnar F. Kaufmann 11. Imaging N-Acyl Homoserine Lactone Quorum Sensing In vivo Louise Dahl Christensen, Maria van Gennip, Tim Holm Jakobsen, Michael Givskov, and Thomas Bjarnsholt 12. Defining the Structure and Function of Acyl-Homoserine Lactone Autoinducers Mair E.A. Churchill, Hiruy M. Sibhatu, and Charis L. Uhlson 13. Global Expression Analysis of Quorum-Sensing Controlled Genes Martin Schuster 14. Small RNA Target Genes and Regulatory Connections in the Vibrio cholerae Quorum Sensing System Brian K. Hammer and Sine Lo Svenningsen 15. Quantifying Pseudomonas aeruginosa Quinolones and Examining Their Interactions with Lipids Gregory C. Palmer, Jeffrey W. Schertzer, Lauren Mashburn-Warren, and Marvin Whiteley 16. Linking Quorum Sensing Regulation and Biofilm Formation by Candida albicans Aur?lie Deveau and Deborah A. Hogan 17. Design of Synthetic Mammalian Quorum-Sensing Systems Wilfried Weber and Martin Fussenegger Part III: Quorum Sensing Disruption Strategies 18. Qualitative and Quantitative Determination of Quorum Sensing Inhibition In vitro Tim Holm Jakobsen, Maria van Gennip, Louise Dahl Christensen, Thomas Bjarnsholt, and Michael Givskov 19. Custom Synthesis of Autoinducers and Their Analogues Jun Igarashi and Hiroaki Suga 20. Heterologous Overexpression, Purification and in Vitro Characterization of AHL Lactonases Pei W. Thomas and Walter Fast 21. High Performance Liquid Chromatography Analysis of N-Acyl Homoserine Lactone Hydrolysis by Paraoxonases John F. Teiber and Dragomir I. Draganov 22. Generation of Quorum Quenching Antibodies Gunnar F. Kaufmann, Junguk Park, Alexander V. Mayorov, Diane M. Kubitz, and Kim D. Janda

ISBN 1-607619-70-9, ISBN 1-60-761970-9, ISBN 1-60761-970-9, ISBN 1-607-61970-9, ISBN 1-6076-1970-9

ISBN 978-1-607619-70-3, ISBN 978-1-60-761970-3, ISBN 978-1-60761-970-3, ISBN 978-1-607-61970-3, ISBN 978-1-6076-1970-3

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