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Wu, Catherine J. (Herausgeber)

Protein Microarray for Disease Analysis Methods and Protocols (Gebundene Ausgabe)

Reihe: Methods in Molecular Biology 723

Springer-Verlag GmbH, Humana Press, November 2011

373 S. - Sprache: Englisch - 64 schwarz-weiße und 8 farbige Abbildungen, 11 schwarz-weiße Tabellen - 262x187x30 mm Book

ISBN: 1617790427 EAN: 9781617790423

Protein microarrays have been used for a wide variety of important tasks, such as identifying protein-protein interactions, discovering disease biomarkers, identifying DNA-binding specificity by protein variants, and for characterization of the humoral immune response.  In Protein Microarray for Disease Analysis: Methods and Protocols, expert researchers provide concise descriptions of the methodologies currently used to fabricate microarrays for the comprehensive analysis of proteins or responses to proteins that can be used to dissect human disease.  These methodologies are the toolbox for revolutionizing drug development and cell-level biochemical understanding of human disease processes.  Beginning with a section on protein-detecting analytical microarrays, the volume continues with sections covering antigen microarrays for immunoprofiling, protein function microarrays, the validation of candidate targets, proteomic libraries, as well as signal detection strategies and data analysis techniques.  Written in the highly successful Methods in Molecular Biology(TM) series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.  Practical and cutting-edge,  Protein Microarray for Disease Analysis: Methods and Protocols serves as a solid framework to aid scientists in understanding how protein microarray technology is presently developing and how it can be applied to transform our analysis of human disease.

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Serves as a toolbox of methodologies currently used in the field of protein microarray studyFeatures step-by-step protocols ready for the labContains expert tips and vital implementation advice to ensure success


Part I: Protein-Detecting Analytical Microarrays?1. Detecting and Quantifying Multiple Proteins in Clinical Samples in High-Throughput Using Antibody Microarrays??????????? Tanya Knickerbocker and Gavin MacBeath?2. Analysis of Serum Protein Glycosylation with Antibody-Lectin Microarray for High-Throughput Biomarker Screening??????????? Chen Li and David M. Lubman?3. Antibody Suspension Bead Arrays??????????? Jochen M. Schwenk and Peter Nilsson?4. Reverse Protein Arrays Applied to Host-Pathogen Interaction Studies??????????? V?ctor J. Cid, Ekkehard Kauffmann, and Mar?a Molina?5. Identification and Optimization of DNA Aptamer Binding Regions Using DNA Microarrays??????????? Nicholas O. Fischer and Theodore M. Tarasow?6. Recombinant Lectin Microarrays for Glycomic Analysis??????????? Daniel C. Propheter, Ku-Lung Hsu, and Lara K. Mahal?Part II: Antigen Microarrays for Immunoprofiling?7. Recombinant Antigen Microarrays for Serum/Plasma Antibody Detection??????????? Persis P. Wadia, Bita Sahaf, and David B. Miklos?8. SPOT Synthesis as a Tool to Study Protein-Protein Interactions??????????? Dirk F.H. Winkler, Heiko Andresen, and Kai Hilpert?9. Native Antigen Fractionation Protein Microarrays for Biomarker Discovery??????????? Robert J. Caiazzo, Jr., Dennis J. O'Rourke, Timothy J. Barder, Bryce P. Nelson, and Brian C.-S. Liu?10. Immunoprofiling Using NAPPA Protein Microarrays??????????? Sahar Sibani and Joshua LaBaer?Part III: Protein Function Microarrays?11. High-Throughput Mammalian Two-Hybrid Screening for Protein-Protein Interactions Using Transfected Cell Arrays (CAPPIA)??????????? Andrea Fiebitz and Dominique Vanhecke?12. Protein-Protein Interactions: An Application of Tus-Ter Mediated Protein Microarray System??????????? Kalavathy Sitaraman and Deb K. Chatterjee?13. Kinase Substrate Interactions??????????? Michael G. Smith, Jason Ptacek, and Michael Snyder?14. A Functional Protein Microarray Approach to Characterizing Posttranslational Modifications on Lysine Residues??????????? Jun Seop Jeong, Hee-Sool Rho, and Heng Zhu?Part IV: Strategies for Validation of Candidate Targets?15. Multiplexed Detection of Antibodies Using Programmable Bead Arrays??????????? Karen S. Anderson?16. A Co-Precipitation-Based Validation Methodology for Interactions Identified Using Protein Microarrays??????????? Ovidiu Marina,? Jonathan S. Duke-Cohan, and Catherine J. Wu?Part V: Generation of Proteomic Libraries?17. Development of Expression-Ready Constructs for Generation of Proteomic Libraries??????????? Charles Yu, Kenneth H. Wan, Ann S. Hammonds, Mark Stapleton, Joseph W. Carlson, and Susan E. Celniker?Part VI: Detection Methods?18. Reverse Phase Protein Microarrays: Fluorometric and Colorimetric Detection??????????? Rosa I. Gallagher, Alessandra Silvestri, Emanuel F. Petricoin III, Lance A. Liotta, and Virginia Espina?19. F?rster Resonance Energy Transfer Methods for Quantification of Protein-Protein Interactions on Microarrays??????????? Michael Sch?ferling and Stefan Nagl?20. Label-Free Detection with Surface Plasmon Resonance Imaging??????????? Christopher Lausted,? Zhiyuan Hu, and Leroy Hood?Part VII: Data Analysis Techniques for Protein Function Microarrays?21. Data Processing and Analysis for Protein Microarrays??????????? David S. DeLuca, Ovidiu Marina, Surajit Ray, Guang L. Zhang, Catherine J. Wu, and Vladimir Brusic?22. Database Resources for Proteomics-Based Analysis of Cancer??????????? Guang Lan Zhang, David S. DeLuca, and Vladimir Brusic


From the reviews:"This is a well-illustrated book with concise descriptions of the methodologies currently used to fabricate microarrays for the comprehensive analysis of proteins or responses to proteins that can be used to detect disease. ... The book is intended for scientists, but it also will be of immense help to graduate students and researchers interested protein microarrays. ... a well-done resource on the methodologies currently used in protein microarrays to promote a cell-level biochemical understanding of human disease processes that could revolutionize drug development." (Omer Iqbal, Doody's Review Services, July, 2011)

ISBN 1-617790-42-7, ISBN 1-61-779042-7, ISBN 1-61779-042-7, ISBN 1-617-79042-7, ISBN 1-6177-9042-7

ISBN 978-1-617790-42-3, ISBN 978-1-61-779042-3, ISBN 978-1-61779-042-3, ISBN 978-1-617-79042-3, ISBN 978-1-6177-9042-3

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