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Li, Ka Wan (Herausgeber)

Neuroproteomics (Gebundene Ausgabe)

Reihe: Neuromethods 57

Springer-Verlag GmbH, Humana Press Inc., September 2011

317 S. - Sprache: Englisch - 59 schwarz-weiße und 17 farbige Abbildungen, 24 schwarz-weiße Tabellen - 266x187x25 mm Book

ISBN: 1617791105 EAN: 9781617791109

In the past few years, the application of proteomics to examine the molecular mechanisms underlying (mal-)functioning of the nervous system and brain disorders has risen steeply, which in many cases has yielded novel insights. As neuroproteomics is a new research field involving the use of a number of high-end analytical instruments and technologies, both promises and pitfalls may not be well appreciated by the researchers, and the design of a proper proteomics experiment may not be obvious.  Neuroproteomics brings together a collection of detailed protocols written by experienced professionals in the field in order to cover aspects of various dimensions of this new technology, together with some established methods that have supporting roles in the workflow of neuroproteomics. Written for the popular Neuromethods series, the chapters in this book contain step-by-step, easy to follow instruction along with practical tips and key implementation advice essential for successful implementation of the techniques.   Authoritative and cutting-edge, Neuroproteomics will be of great assistance to all researchers seeking to design and execute their own proteomics experiments in an optimal way and continue moving forward this exciting area of study.

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Covers both techniques involving cutting-edge technology and more established methodsFeatures clear, practical protocols for readily reproducible resultsServes as an ideal guide to the designing of successful neuroproteomics experiments


Part I: Introduction?1. Neuroproteomics: Deciphering Brain Function and Disorders??????????? Ka Wan Li?Part II: Fractionation of Brain Regions, Organelles, and Protein Complexes?2. Dissection of Rodent Brain Regions??????????? Sabine Spijker?3. Subcellular Fractionation of Brain Tissue Using Free Flow Electrophoresis??????????? Markus Islinger, Joachim Kirsch, Sabine Angerm?ller, Ramona Rotaru, Afsaneh Abdolzade-Bavil, and Gerhard Weber?4. Isolation of Synapse Sub-Domains by Subcellular Fractionation Using Sucrose Density Gradient Centrifugation??????????? Tatsuo Suzuki?5. Enrichment of Plasma Membranes from Small Brain Samples by Aqueous Polymer Two-Phase Systems??????????? Jens Schindler?6. Identification and Characterization of Protein Complexes from Total Brain and Synaptoneurosomes: Heterogeneity of Molecular Complexes in Distinct Subcellular Domains??????????? Silvia De Rubeis and Claudia Bagni?7. Analysis of Protein Complexes by 2D Blue Native/SDS-PAGE and Antibody-Shift Assay??????????? Dong Yang, Xinyu Deng, Ying Jiang, and Fuchu He?Part III: Analytical Tools for Neuroproteomics?8. 2-Dimensional Gel Electrophoresis-Based Proteomic Analysis of Brain Synapses??????????? Karl-Heinz Smalla and Ursula Wyneken?9. 2-Dimensional BAC-SDS Polyacrylamide Gel Electrophoresis for the Fractionation and Identification of Synaptic Vesicle Proteins and the Presynaptic Active Zone??????????? Joern Barth and Walter Volknandt?10. iTRAQ-Based LC-LC MALDI TOF/TOF MS Quantitative Analysis of Membrane Proteins from Human Glioma??????????? UroS Rajcevic?11. OFFGEL-Isoelectric Focusing Fractionation for the Analysis of Complex Proteome??????????? Emilie Ernoult and Catherine Guette?12. A 1D-PAGE/LC-ESI Linear Ion Trap Orbitrap MS Approach for the Analysis of Synapse Proteomes and Synaptic Protein Complexes??????????? Ning Chen, Roel C. vd Schors and August B. Smit?13. SDS-PAGE Immuno-Blot Analysis??????????? Patricia Klemmer?14. Phosphoproteomics by Highly Selective IMAC Protocol??????????? Chia-Feng Tsai, Yi-Ting Wang, Pei-Yi Lin, and Yu-Ju Chen?15. Global Analysis of Ubiquitination??????????? David Meierhofer and Peter Kaiser?16. High-Throughput High-Content Functional Image Analysis of Neuronal Proteins Implicated in Parkinson's Disease??????????? Eva Blaas and Ronald E. van Kesteren?Part IV: Analysis of Specific Brain Tissues and Fluid?17. Neuropeptidomics of the Mammalian Brain??????????? Fang Xie, Elena V. Romanova, and Jonathan V. Sweedler?18. In-Depth Analysis of the Cerebrospinal Fluid Proteome and Biomarker Discovery: Abundant Protein Depletion Sample Pretreatment Method??????????? Silvina A. Fratantoni and Connie R. Jimenez?19. Lipidomics of the Nervous System: Phospholipidomics by Liquid Chromatography Coupled to Mass Spectrometry or Tandem Mass Spectrometry??????????? Su Chen?Part V: Bioinformatics?20. Bioinformatics Procedures for Analysis of Quantitative Proteomics Experiments Using iTRAQ??????????? Pim van Nierop and Maarten Loos?21. Statistical Analysis of Spectral Count Data Generated by Label-Free Tandem Mass Spectrometry-Based Proteomics??????????? Thang V. Pham and Connie R. Jimenez

ISBN 1-617791-10-5, ISBN 1-61-779110-5, ISBN 1-61779-110-5, ISBN 1-617-79110-5, ISBN 1-6177-9110-5

ISBN 978-1-617791-10-9, ISBN 978-1-61-779110-9, ISBN 978-1-61779-110-9, ISBN 978-1-617-79110-9, ISBN 978-1-6177-9110-9

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