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Al-Mulla, Fahd (Herausgeber)

Formalin-Fixed Paraffin-Embedded Tissues Methods and Protocols (Gebundene Ausgabe)


Reihe: Methods in Molecular Biology 724

Springer-Verlag GmbH, Februar 2011


312 S. - Sprache: Englisch - 56 schwarz-weiße und 1 farbige Abbildungen, 13 schwarz-weiße Tabellen - 265x187x25 mm Book

ISBN: 1617790540 EAN: 9781617790546

Presenting an area of research that intersects with and integrates diverse disciplines, including genomics, epigenetics, proteomics, and cellular biology, among others, Formalin-Fixed Paraffin-Embedded Tissues: Methods and Protocols collects contributions from expert researchers in order to provide practical guidelines to this complex study. Compiled in order to provide researchers with up-to-date methodological information pertaining to the utilization of genomic, transcriptomic, and proteomic data in diagnosis, prognosis, and tailored therapy, the ultimate aim of this volume is to decipher diseases at a molecular level. Divided into multiple convenient chapters, this detailed book covers various techniques to construct and utilize tissue arrays, it also provides detailed protocols in immunohistochemistry, immunofluorescence, fluorescent and chromogenic in situ hybridization, and ultimately introduces protocols for FFPET microdissection and nucleic acids extraction for their utilization in advanced techniques such as microarray CGH, DNA methylation and pyrosequencing. The volume also discusses FFPET research from an ethical standpoint and concludes with a chapter on novel tissue fixative. Written in the highly successful Methods in Molecular Biology(TM) series format, chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls. ? Authoritative and accessible, Formalin-Fixed Paraffin-Embedded Tissues: Methods and Protocols serves as a practical guide for scientists of all backgrounds and aims to convey the appropriate sense of fascination associated with this vital field of research.


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Produktdetails

Includes well-established and tested methods and protocolsProvides step-by-step detail essential for reproducible resultsContains well-organized themes and key implementation advice from the experts

Inhaltsverzeichnis

1. Regulatory and Ethical Issues on the Utilization of FFPE Tissues in Research??????????? Catherine M. With, David L. Evers, and Jeffrey T. Mason?2.? Tissue Microarrays: Construction and Uses??????????? Carol B. Fowler, Yan-Gao Man, Shimin Zhang, Timothy J. O'Leary, Jeffrey T. Mason, and Robert E. Cunningham?3. Standardization in Immunohistology ??????????? Anthony S-Y Leong and Trishe Y-M Leong?4. Multiple Immunofluorescence Labeling of Formalin-fixed Paraffin-embedded (FFPE) Tissue ??????????? David Robertson and Clare M. Isacke?5. Microwaves for Chromogenic-in-situ-hybridization ??????????? Anthony S-Y Leong and Zenobia Haffajee?6. Automated Analysis of FISH-stained HER2/neu Samples with Metafer ??????????? Christian Schunck and Eiman Mohammad?7. Laser Capture Microdissection (LCM) of FFPE Tissue SectionsBridging the Gap between Microscopy and Molecular Analysis ??????????? Renate Burgemeister?8. Nucleic Acids Extraction from Laser Microdissected FFPE Tissue Sections ??????????? Renate Burgemeister?9. Microarray-based CGH and Copy Number Analysis of FFPE Samples??????????? Fahd Al-Mulla?10. Microarray Profiling of DNA Extracted from FFPE Tissues using SNP6.0 Affymetrix Platform ??????????? Marianne Tuefferd, An de Bondt, Ilse Van den Wyngaert, Willem Talloen, and Hinrich G?hlmann?11. Whole Genome Amplification of DNA Extracted From FFPE Tissues??????????? Mira Bosso and Fahd Al-Mulla?12. Pyrosequencing of DNA Extracted from Formalin-Fixed Paraffin-embedded Tissue ?????? ??????????? Brendan Doyle, Ciar?n O'Riain, and Kim Appleton?13. Analysis of DNA Methylation in? FFPE Tissues using the MethyLight Technology??????????? ??????????? Ashraf Dallol, Waleed Al- Ali, Amina Al-Shaibani, and Fahd Al-Mulla?14. RT-PCR Gene Expression Profiling of RNA from Paraffin-Embedded Tissues Prepared Using a Range of Different Fixatives and Conditions ??????????? Mei-Lan Liu, Jennie Jeong, Ranjana Ambannavar, Carl Millward, Frederick Baehner, Chithra Sangli, Debjani Dutta, Mylan Pho, Anhthu Nguyen, and Maureen T. Cronin?15. RT-PCR-Based Gene Expression Profiling for Cancer Biomarker Discovery from Fixed Paraffin Embedded Tissues ??????????? Aaron Scott, Ranjana Ambannavar, Jennie Jeong, Mei-Lan Liu and Maureen T. Cronin?16. microRNA Isolation from Formalin-Fixed Paraffin-Embedded Tissues??????????? Aihua Liu and Xiaowei Xu?17. Gene Expression Profiling of RNA Extracted from FFPE tissues:? NuGEN Technologies' Whole-Transcriptome Amplification System ??????????? Leah Turner, Joe Don Heath, and Nurith Kurn?18. Protein Mass Spectrometry Applications on FFPE Tissue Sections ??????????? Carol B. Fowler, Timothy J. O'Leary, and Jeffrey T. Mason?19. An Alternative Fixative to Formalin Fixation for Molecular Applications: the RCL2?-CS100 Approach??????????? Am?lie Denou?l, Florence Boissi?re-Michot, Philippe Rochaix, Fr?d?ric Bibeau, Nathalie Boulle

Kritikerstimmen

From the reviews: "This text can be considered an introductory compendium of current methods and protocols in Molecular Biology for the use of Fixed Paraffin-Embedded Tissues that will be particularly suited to new researchers in the field who are eager to develop expertise. It is an easily readable and technically comprehensive book that would be ideal for initial experimental planning which can be complemented by other bibliographic sources and scientific journals for in-depth discussions of particular techniques and their potential applications." (Flavia Troglio and Manuela Vecchi, Molecular Biotechnology, 2012)


ISBN 1-617790-54-0, ISBN 1-61-779054-0, ISBN 1-61779-054-0, ISBN 1-617-79054-0, ISBN 1-6177-9054-0

ISBN 978-1-617790-54-6, ISBN 978-1-61-779054-6, ISBN 978-1-61779-054-6, ISBN 978-1-617-79054-6, ISBN 978-1-6177-9054-6

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