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Zur Nieden, Nicole I. (Herausgeber)

Embryonic Stem Cell Therapy for Osteo-Degenerative Diseases Methods and Protocols (Gebundene Ausgabe)

Reihe: Methods in Molecular Biology 690

Springer-Verlag GmbH, Humana Press, November 2010

336 S. - Sprache: Englisch - 65 schwarz-weiße und 65 farbige Abbildungen, 12 schwarz-weiße Tabellen - 270x186x25 mm

ISBN: 160761961X EAN: 9781607619611

Embryonic stem cells (ESCs) offer an unlimited self-renewing capacity, as opposed to the limits of adult stem cells; therefore, ESCs represent an almost bottomless resource for regenerative medicine and tissue engineering approaches. In Embryonic Stem Cell Therapy for Osteo-Degenerative Diseases: Methods and Protocols, accomplished investigators provide detailed descriptions on how to expand ESCs from the most commonly used species ex vivo, i.e. mouse and human, in static culture as well as in controllable bioreactor processes. The thorough and timely volume summarizes the methods that may be used to differentiate these cells along the desired lineage of choice, be it osteoblasts, osteoclasts, or chondrocytes, and consequentially also offers analysis tools for the characterization of resulting cells and evaluation of differentiation effectiveness. Written in the highly successful Methods in Molecular Biology(TM) series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and expert tips on troubleshooting and avoiding known pitfalls. Dependable and cutting-edge, Embryonic Stem Cell Therapy for Osteo-Degenerative Diseases: Methods and Protocols supplies the tools necessary to allow researchers to carry out critical research needed in order to bring this burgeoning and vitally important field closer to the clinic and to ensure the widespread application of a successful strategy.

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Contains detailed, expert protocols specific to ESCs and stem cell differentiation Gives special care to address possible methodological pitfalls and provides trouble-shooting guides in the form of Notes sections Features contributions from renowned researchers in the field of stem cell self-renewal and the pioneers in skeletogenesis from ESCs


1. Embryonic Stem Cells for Osteo-Degenerative Diseases Nicole I. zur Nieden 2. Methods for Culturing Mouse and Human Embryonic Stem Cells Sabrina Lin and Prue Talbot 3. Serum-Free and Feeder free Culture Conditions for Human Embryonic Stem Cells Ludovic Vallier 4. Functional Assays for Human Embryonic Stem Cell Pluripotency Michael D. O'Connor, Melanie D. Kardel, and Connie J. Eaves 5. Using Cadherin Expression to Assess Spontaneous Differentiation of Embryonic Stem Cells Helen Spencer, Maria Keramari, and Christopher M. Ward 6. Generation of Human Embryonic Stem Cells Carrying Lineage Specific Reporters Parinya Noisa, Alai Urrutikoetxea-Uriguen, and Wei Cui 7. Manipulations of MicroRNA in Human Pluripotent Stem Cells and Their Derivatives Stephanie N. Rushing, Anthony W. Herren, Deborah K Lieu, and Ronald A. Li 8. Large-Scale Expansion of Mouse Embryonic Stem Cells on Microcarriers Ana M. Fernandes, Maria Margarida Diogo, Cl?udia Lobato da Silva, and Joaquim M.S. Cabral 9. Embryoid Body Formation: Recent Advances in Automated Bioreactor Technology Susanne Trettner, Alexander Seeliger, and Nicole I. zur Nieden 10. Methods for Embryoid Body Formation: The Microwell Approach Dawn P. Spelke, Daniel Ortmann, Ali Khademhosseini, Lino Ferreira, and Jeffrey M. Karp 11. Human Embryonic Stem Cell-Derived Mesenchymal Progenitors: An Overview Peiman Hematti 12. Derivation of Mesenchymal Stem Cells from Human Embryonic Stem Cells Andre Choo and Sai Kiang Lim 13. Differentiation of Human Embryonic Stem Cells into Mesenchymal Stem Cells by the "Raclure" Method Emmanuel N. Olivier and Eric E. Bouhassira 14. Improved Media Compositions for the Differentiation of Embryonic Stem Cells into Osteoblasts and Chondrocytes Beatrice Kuske, Vuk Savkovic, and Nicole I. zur Nieden 15. Differentiation of Mouse Embryonic Stem Cells in Self-Assembling Peptide Scaffolds N?ria Mar?-Buy? and Carlos E. Semino 16. Methods for Investigation of Osteoclastogenesis Using Mouse Embryonic Stem Cells Motokazu Tsuneto, Toshiyuki Yamane, and Shin-Ichi Hayashi 17. Absorption-Based Assays for the Analysis of Osteogenic and Chondrogenic Yield Lesley A. Davis, Anke Dienelt, and Nicole I. zur Nieden 18. Identification of Osteoclasts in Culture Nobuyuki Udagawa, Teruhito Yamashita, Yasuhiro Kobayashi, and Naoyuki Takahashi 19. Analysis of Glycosaminoglycans in Stem Cell Glycomics Boyangzi Li, Haiying Liu, Zhenqing Zhang, Hope E. Stansfield, Jonathan S. Dordick, and Robert J. Linhardt 20. Drill Hole Defects: Induction, Imaging, and Analysis in the Rodent Andre Obenaus and Pedro Hayes 21. Measurement and Illustration of Immune Interaction after Stem Cell Transplantation Stephan Fricke


From the reviews:"Provides step-by-step protocols for the differentiation of ESCs along the musculoskeletal lineage. ... This book is written for clinicians and basic science researchers interested in development of the musculoskeletal system and the promise of stem cells for treating patients with debilitating osteodegenerative diseases. ... The authors provide excellent minireviews and great easy-to-follow research protocols. ... The illustrations are educational and motivating. I recommend this book for research scientists interested in new approaches for treating patients with osteodegenerative diseases." (Bruce A. Fenderson, Doody's Review Service, June, 2011)

ISBN 1-607619-61-X, ISBN 1-60-761961-X, ISBN 1-60761-961-X, ISBN 1-607-61961-X, ISBN 1-6076-1961-X

ISBN 978-1-607619-61-1, ISBN 978-1-60-761961-1, ISBN 978-1-60761-961-1, ISBN 978-1-607-61961-1, ISBN 978-1-6076-1961-1

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