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Tsubouchi, Hideo (Herausgeber)

DNA Recombination Methods and Protocols (Gebundene Ausgabe)

Reihe: Methods in Molecular Biology 745

Springer-Verlag GmbH, Humana Press, Juni 2012

565 S. - Sprache: Englisch - 105 schwarz-weiße und 3 farbige Abbildungen, 15 schwarz-weiße Tabellen - 262x189x38 mm Book

ISBN: 1617791288 EAN: 9781617791284

Homologous recombination is important in various aspects of DNA metabolism, including damage repair, replication, telomere maintenance, and meiosis, and yeast genetics has successfully provided a framework for the mechanism of homologous recombination. Divided into four convenient sections, DNA Recombination: Methods and Protocols covers recent techniques that best utilize the advantages of the yeast system, prescribing to the belief that yeast will keep serving as a great model organism to study homologous recombination. Chapters have also been included for such exceptions as the group of genes involved in recombination that are found solely in higher eukaryotes, such as BRCA2. And looking forward, a necessary step in the direction of understanding the homologous recombination process is to isolate the machine and let it work in a test tube.  Understanding the design by studying the appearance and behavior of the machinery as a single molecule will be an important milestone toward understanding the mechanism of action of the machinery. Techniques covering these topics have also been included.  Written in the successful Methods in Molecular Biology(TM) series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.   Authoritative and easily accessible, DNA Recombination: Methods and Protocols serves as an ideal guide to scientists of all backgrounds with its well-honed methodologies and strives to bring the reader to the next level of understanding regarding this vital subject.

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Part I. Genetic and Molecular Biological Approaches with Yeast?1. Methods to Study Mitotic Homologous Recombination and Genome Stability??????????? Xiuzhong Zheng, Anastasiya Epstein, and Hannah L. Klein?2. Characterizing Resection at Random and Unique Chromosome Double-Strand Breaks and Telomere Ends??????????? Wenjian Ma, Jim Westmoreland, Wataru Nakai, Anna Malkova, and Michael A. Resnick?3. Characterization of Meiotic Recombination Initiation Sites Using Pulsed-field Gel Electrophoresis??????????? Sarah Farmer, Wing-Kit Leung, and Hideo Tsubouchi?4. Genome-Wide Detection of Meiotic DNA Double-Strand Break Hotspots Using Single-stranded DNA??????????? Hannah G. Blitzblau and Andreas Hochwagen?5. Detection of Covalent DNA-Bound Spo11 and Topoisomerase Complexes??????????? Edgar Hartsuiker?6. Molecular Assays to Investigate Chromatin Changes during DNA Double-Strand Break Repair in Yeast??????????? Scott Houghtaling, Toyoko Tsukuda, and Mary Ann Osley?7. Analysis of Meiotic Recombination Intermediates by Two-Dimensional Gel Electrophoresis??????????? Jasvinder A. Singh and G. Valentin B?rner?8. Mapping of Crossover Sites Using DNA Microarrays??????????? Stacy Y. Chen and Jennifer C. Fung?9. Using the Semi-Synthetic Epitope System to Identify Direct Substrates of the Meiosis-Specific Budding Yeast Kinase, Mek1??????????? Hsiao-Chi Lo and Nancy M. Hollingsworth?10. Genetic and Molecular Analysis of Mitotic Recombination in Saccharomyces cerevisiae??????????? Bel?n G?mez-Gonz?lez, Jos? F. Ruiz, and Andr?s Aguilera?11. In vivo Site-Specific Mutagenesis and Gene Collage Using the Delitto Perfetto System in Yeast Saccharomyces cerevisiae??????????? Samantha Stuckey, Kuntal Mukherjee, and Francesca Storici?12. Detection of RNA-Templated Double-Strand Break Repair in Yeast??????????? Ying Shen and Francesca Storici?Part II. Genetic and Molecular Biological Approaches with Higher Eukaryotes?13. SNP-Based Mapping of Crossover Recombination in C. elegans??????????? Grace C. Bazan and Kenneth J. Hillers?14. Characterization of Meiotic Crossovers in Pollen from Arabidopsis thaliana??????????? Jan Drouaud and Christine M?zard?15. Isolation of Meiotic Recombinants from Mouse Sperm??????????? Francesca Cole and Maria Jasin?16. Homologous Recombination Assay for Interstrand Crosslink Repair??????????? Koji Nakanishi, Francesca Cavallo, Erika Brunet, and Maria Jasin?17. Evaluation of Homologous Recombinational Repair in Chicken B Lymphoma Cell Line, DT40??????????? Hiroyuki Kitao, Seiki Hirano, and Minoru Takata?18. Understanding the Immunoglobulin Locus Specificity of Hypermutation??????????? Vera Batrak, Artem Blagodatski, and Jean-Marie Buerstedde?Part III.? In Vitro Reconstitution of Homologous Recombination Reactions and Single Molecular Analysis of Recombination Proteins?19. Quality Control of Purified Proteins Involved in Homologous Recombination??????????? Xiao-Ping Zhang and Wolf-Dietrich Heyer?20. Assays for Structure-Selective DNA Endonucleases??????????? William D. Wright, Kirk T. Ehmsen, and Wolf-Dietrich Heyer?21. In vitro Assays for DNA Pairing and ?Recombination-Associated DNA Synthesis??????????? Jie Liu, Jessica Sneeden, and Wolf-Dietrich Heyer?22. An In vitro Assay for Monitoring the Formation and Branch Migration of Holliday Junctions Mediated by a Eukaryotic Recombinase??????????? Yasuto Murayama and Hiroshi Iwasaki?23. Reconstituting the Key Steps of the DNA Double-Strand Break Repair In vitro ??????????? Matthew J. Rossi, Dmitry V. Bugreev, Olga M. Mazina, and Alexander V. Mazin?24. Biochemical Studies on Human Rad51-Mediated Homologous Recombination??????????? Youngho Kwon, Weixing Zhao, and Patrick Sung?25. Studying DNA Replication Fork Stability in Xenopus Egg Extract??????????? Yoshitami Hashimoto and Vincenzo Costanzo?26. Supported Lipid-Bilayers and DNA Curtains for High-Throughput Single Molecule Studies??????????? Ilya J. Finkelstein and Eric C. Greene?27. FRET-Based Assays to Monitor DNA Binding and Annealing by Rad52 Recombination Mediator Protein ??????????? Jill M. Grimme and Maria Spies?28. Visualization of Human Dmc1 Presynaptic Filaments??????????? Michael G. Sehorn and Hilarie A. Sehorn?Part IV.? Cell Biological Approaches to Study the In Vivo Behavior of Homologous Recombination?29. Tracking of Single and Multiple Genomic Loci in Living Yeast Cells??????????? Imen Lassadi and Kerstin Bystricky?30. Cell Biology of Homologous Recombination in Yeast??????????? Nadine Eckert-Boulet, Rodney Rothstein, and Michael Lisby?31. Live Cell Imaging of Meiotic Chromosome Dynamics in Yeast ??????????? Harry Scherthan and Caroline Adelfalk?32. Chromosome Structure and Homologous Chromosome Association during Meiotic Prophase in C. elegans??????????? Kentaro Nabeshima

ISBN 1-617791-28-8, ISBN 1-61-779128-8, ISBN 1-61779-128-8, ISBN 1-617-79128-8, ISBN 1-6177-9128-8

ISBN 978-1-617791-28-4, ISBN 978-1-61-779128-4, ISBN 978-1-61779-128-4, ISBN 978-1-617-79128-4, ISBN 978-1-6177-9128-4

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