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Zuccheri, Giampaolo (Herausgeber); Samorì, Bruno (Herausgeber)

DNA Nanotechnology Methods and Protocols (Gebundene Ausgabe)

Reihe: Methods in Molecular Biology 749

Springer-Verlag GmbH, Humana Press, August 2011

361 S. - Sprache: Englisch - 37 schwarz-weiße Abbildungen, 7 schwarz-weiße Tabellen - 261x185x28 mm

ISBN: 1617791415 EAN: 9781617791413

Currently, nanotechnology is exposing the properties of DNA in unprecedented detail leading to new insights on the biological behavior and function of DNA. With the structural perfection of a self-assembling DNA nano-object, such as a DNA origami, it is clear how complex DNA is as a molecule, leading researchers to wonder how many different constructs could be designed and realized. DNA Nanotechnology: Methods and Protocols shows the procedures to follow in order to repeat methods that lead to such constructs or to the mastering of the characterization techniques used to study them. The chapters of this book are roughly divided into two parts: some cover the methods for preparing the nanostructures, from the rationale of the operations to the techniques for their handling, while other chapters deal more directly with advanced instrumental techniques that can manipulate and characterize molecules and nanostructures. Written in the highly successful Methods in Molecular Biology(TM) series format, chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and accessible, DNA Nanotechnology: Methods and Protocols serves as an ideal guide to scientists of all backgrounds and aims to ignite interest and spur activity in this young and rapidly growing research field.Includes cutting-edge methods and protocolsProvides step-by-step detail essential for reproducible resultsContains key notes and implementation advice from the experts

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Includes cutting-edge methods and protocolsProvides step-by-step detail essential for reproducible resultsContains key notes and implementation advice from the experts


1. Synthesis and Characterization of Self-assembled DNA Nanostructures ??????????? Chenxiang Lin, Yonggang Ke, Rahul Chhabra, Jaswinder Sharma, Yan Liu, and Hao Yan?2. Protocols for Self-assembly and Imaging of DNA Nanostructures ??????????? Thomas L. Sobey and Friedrich C. Simmel?3. Self-Assembly of Metal-DNA Triangles and DNA Nanotubes with Synthetic Junctions??????????? Hua Yang, Pik Kwan Lo, Christopher K. McLaughlin, Graham D. Hamblin, Faisal A. Aldaye, and Hanadi F. Sleiman?4. DNA-Templated? Pd Conductive Metallic Nanowires ??????????? Khoa Nguyen, Stephane Campidelli, and Arianna Filoramo?5. A Method to Map Spatiotemporal pH Changes Inside Living Cells using a pH Triggered DNA Nanoswitch??????????? Souvik Modi and Yamuna Krishnan?6. Control of Helical Handedness in DNA and PNA Nanostructures??????????? Roberto Corradini, Tullia Tedeschi, Stefano Sforza, Mark M. Green, and Rosangela Marchelli?7. G-quartet, G-quadruplex, and G-wire Regulated by Chemical Stimuli ??????????? Daisuke Miyoshi and Naoki Sugimoto?8. Preparation and Atomic Force Microscopy of Quadruplex DNA??????????? James Vesenka?9. Synthesis of Long DNA-based Nanowires??????????? Alexander Kotlyar ?10. G-wire Synthesis and Modification with Gold-nanoparticle ??????????? Christian Leiterer, Andrea Csaki, and Wolfgang Fritzsche?11. Preparation of DNA Nanostructures with Repetitive Binding Motifs by Rolling Circle Amplification ??????????? Edda Rei?, Ralph H?lzel, and Frank F. Bier?12. Controlled Confinement of DNA at the Nanoscale: Nanofabrication and Surface Bio-functionalization ??????????? Matteo Palma, Justin Abramson, Alon A. Gorodetsky, Colin Nuckolls, Michael P. Sheetz, Shalom J. Wind, and James Hone?13. Templated Assembly of DNA Origami Gold Nanoparticle Arrays on Lithographically Patterned Surfaces ??????????? Albert M. Hung and Jennifer N. Cha?14. DNA-modified Single Crystal and Nanoporous Silicon??????????? Andrew Houlton, Bernard A. Connolly, Andrew R. Pike, and Benjamin R. Horrocks?15. The Atomic Force Microscopy as a Lithographic Tool: Nanografting of DNA Nanostructures for Biosensing Applications ??????????? Matteo Castronovo and Denis Scaini?16. Trapping and Immobilization of DNA Molecules Between Nanoelectrodes ??????????? Anton Kuzyk, J. Jussi Toppari, and P?ivi T?rm?17. DNA Contour Length Measurements as a Tool for the Structural Analysis of DNA and Nucleoprotein Complexes??????????? Claudio Rivetti?18. DNA Molecular Handles for Single Molecule Protein Folding Studies by Optical Tweezers ??????????? Ciro Cecconi, Elizabeth A. Shank, Susan Marqusee, and Carlos Bustamante?19. Optimal Practices for Surface-tethered Single Molecule Total Internal Reflection Fluorescence Resonance Energy Transfer Analysis ??????????? Matt V. Fagerburg and Sanford H. Leuba?20. Engineering Mononucleosomes for Single-Pair FRET Experiments ??????????? Wiepke J.A. Koopmans, Ruth Buning, and John van Noort?21. Measuring DNA-Protein Binding Affinity on a Single Molecule Using Optical Tweezers??????????? Micah J. McCauley and Mark C. Williams?22. Modeling Nanopores for Sequencing DNA??????????? Jeffrey R. Comer, David B. Wells, and Aleksei Aksimentiev

ISBN 1-617791-41-5, ISBN 1-61-779141-5, ISBN 1-61779-141-5, ISBN 1-617-79141-5, ISBN 1-6177-9141-5

ISBN 978-1-617791-41-3, ISBN 978-1-61-779141-3, ISBN 978-1-61779-141-3, ISBN 978-1-617-79141-3, ISBN 978-1-6177-9141-3

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