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Rij, Ronald P. van (Herausgeber)

Antiviral RNAi Concepts, Methods, and Applications (Gebundene Ausgabe)

Reihe: Methods in Molecular Biology 721

Springer-Verlag GmbH, Humana Press, März 2011

Edition. - 411 S. - Sprache: Englisch - 39 schwarz-weiße und 19 farbige Abbildungen, 10 schwarz-weiße Tabellen - 261x185x25 mm Book

ISBN: 1617790362 EAN: 9781617790362

Viruses and RNAi share an intricate relationship at many levels. RNAi is an important antiviral defense mechanism in plants and invertebrates, microRNAs - of viral or cellular origin - affect many aspects of virus biology, and replication of many, if not all, mammalian viruses can be suppressed by RNAi. Antiviral RNAi: Concepts, Methods, and Applications provides a collection of protocols for the analysis of viral small RNAs and natural antiviral RNAi responses as well as for the development and optimization of RNAi-based antiviral drugs. As RNAi is a central regulatory mechanism in the cell, the methods in this volume can also be applied out of the context of a virus infection. Divided into five convenient parts, this detailed volume reviews important basic concepts in the field of antiviral RNAi, provides experimental and bio-informatic tools for the analysis of small silencing RNAs, covers methods to biochemically dissect RNAi-based antiviral defense and viral counter-defense mechanisms, describes methods for the design, expression, and delivery of therapeutic antiviral siRNAs, and finally presents genome-wide RNAi approaches for the identification of factors involved in virus replication. Written in the highly successful Methods in Molecular Biology(TM) series format, chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and accessible, Antiviral RNAi: Concepts, Methods, and Applications serves as an ideal guide for both novice and experienced researchers alike striving to dissect the role of RNAi in the viral life cycle or to further boost the development of novel therapeutics and experimental tools based on RNAi technology.

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Includes cutting-edge methods and protocolsProvides step-by-step detail essential for reproducible resultsContains key notes and implementation advice from the experts


Part I: General Introduction??????????? ??????????? ??????????? ?1. Defense and counter-defense in the RNAi-based antiviral immune system in insects??????????? Jo?l T. van Mierlo, Koen W.R. van Cleef, and Ronald P. van Rij???????????????????????????? ?2. RNAi and cellular miRNAs in infections by mammalian virusesJoost Haasnoot and Ben Berkhout???????????????????????????? ?3. Viral miRNAs??????? Karlie Plaisance-Bonstaff, and Rolf Renne??????????????????????????? ?4. Progress in RNAi-based antiviral therapeutics? Jiehua Zhou and John J. Rossi ?????????????????????????????????? ?5. Chemical modification of small interfering RNA????????? Jesper B. Bramsen and J?rgen Kjems???????????????????????? ??????????? ?Part II: Viral small RNAs?6. Viral small RNA cloning and sequencing?????????? Val?rie Gausson and Maria-Carla Saleh???????????????????? ??????????? 7. Visitor, an informatic pipeline for analysis of viral siRNA sequencing datasets???????? Christophe Antoniewski???????????????????????????????? ?8. Computational prediction of viral miRNAs?????? Adam Grundhoff??????????????????????????????? ?9. Detection of Viral microRNAs by Northern Blot Analysis?????? Lydia V. McClure, Yao-Tang Lin, and Christopher S. Sullivan??????????????????????????????? ?10. Detection of viral microRNA with S1 Nuclease Protection assay?????? Matthias John and S?bastien Pfeffer????????????????????????? ?11. Characterization of RISC associated adenoviral small RNAs ?????????? Ning Xu and G?ran Akusj?rvi??????????????????????????????????? ??????????????????????? ??????????????????????? Part III: RNAi-based antiviral defense ??????????? ??????????????????????????????????? 12. Identification of Viral Suppressors of RNAi by a Reporter Assay in Drosophila S2 Cell CultureKoen W.R. van Cleef, Jo?l T van Mierlo, Marius van den Beek, and Ronald P. van Rij????????????????????????????? 13. Dicer assay in Drosophila S2 cell extract????????? Baojun Yang and Hongwei Li ?????????? ??????????????????????? 14. Slicer activity in Drosophila melanogaster S2 extract? Arabinda Nayak and Raul Andino???????????????? ??????????? 15. Gel Mobility Shift Assays for RNA-binding viral RNAi suppressors??????????? Tibor Csorba and J?zsef Burgy?n?????? ??????????????????????? 16. dsRNA uptake in adult Drosophila?????? Benjamin Obadia and Maria-Carla Saleh??????????????????????????????? ???????????????????????????????????????????????? Part IV: Antiviral RNAi therapy??????????? ??????????????????????????????????? 17. Design of small interfering RNAs for antiviral applications?? Diana Rothe, Erik J. Wade, and Jens Kurreck??????????????????????? ??????????? 18. RNAi inducing lentiviral vectors for anti-HIV-1 gene therapy?????????? Ying Poi Liu, Jan-Tinus Westerink, Olivier ter Brake, and Ben Berkhout???? ??????????????????????? 19. Production of multicopy shRNA lentiviral vectors for antiviral therapy????? Scot D. Henry, Quiwei Pan, and Luc J.W. van der Laan?????? ??????????????????????? 20. Intranasal delivery of antiviral siRNA? Sailen Barik???????????????????????????? ?21. Antibody mediated delivery of siRNAs for anti-HIV therapy??????????? Sang-Soo Kim, Sandesh Subramanya, Dan Peer, Motomu Shimaoka, and Premlata Shankar????????? ??????????????????????? 22. Aptamer-targeted RNAi for HIV-1 therapy??? Jiehua Zhou and John J. Rossi ?????????????????????????????????? ??????????????????????????????????????????????????????????? ??Part V: RNAi screens to study virus-host interactions??????????? ??????????????????????????????????? 23. RNAi screening for host factors involved in viral infection using Drosophila cells? Sara Cherry???????????????????????????? ?24. Genome-wide RNAi Screen for Viral Replication in Mammalian Cell Culture?????? Bhupesh K. Prusty, Alexander Karlas, Thomas F. Meyer, and Thomas Rudel ???????? ??????????????????????? 25. RNAi screening in mammalian cells to identify novel host cell molecules involved in the regulation of viral infections??????? Carolyn B. Coyne and Sara Cherry


From the reviews:"This well-illustrated book details the necessary protocols for the analysis of viral small RNAs and natural antiviral RNAi responses as well as the development and optimization of RNAi-based antiviral drugs. The book is intended for both novice and experienced researchers ... . It also will be of immense help to graduate students, physicians who treat patients with infectious diseases, and other researchers interested in the field of antiviral RNAi. ... a well-illustrated and authoritative resource on important basic concepts in the field of antiviral RNAi." (Omer Iqbal, Doody's Review Service, July, 2011)

ISBN 1-617790-36-2, ISBN 1-61-779036-2, ISBN 1-61779-036-2, ISBN 1-617-79036-2, ISBN 1-6177-9036-2

ISBN 978-1-617790-36-2, ISBN 978-1-61-779036-2, ISBN 978-1-61779-036-2, ISBN 978-1-617-79036-2, ISBN 978-1-6177-9036-2

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