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Zachariou, Michael (Herausgeber)

Affinity Chromatography: Methods and Protocols (Kartoniert / Broschiert / Paperback)


Reihe: Methods in Molecular Biology 421

SPRINGER VERLAG GMBH, Humana Press Inc., November 2010


Softcover of Or - 348 S. - Sprache: Englisch - 231x154x22 mm

ISBN: 1617377015 EAN: 9781617377013

Thirty-eight years after its introduction, affinity chromatography remains a key tool in the armory of separation techniques available to separation and interaction scientists. Expanded and updated from the first edition, this second edition volume aims to provide the beginner with the practical knowledge needed to develop affinity separations suitable for a variety of applications relevant to the post-genomic era. It contains state-of-the-art, brand new protocols, and provides step-by-step laboratory instructions for readily reproducible results.


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Produktdetails

Expanded and updated from the first edition, this second edition volume aims to provide the beginner with the practical knowledge needed to develop affinity separations suitable for a variety of applications relevant to the post-genomic era. It contains state-of-the-art, brand new protocols, and provides step-by-step laboratory instructions for readily reproducible results. Thirty-eight years after its introduction, affinity chromatography remains a key tool in the armory of separation techniques available to separation and interaction scientists.

Inhaltsverzeichnis

PROPOSED TABLE OF CONTENTS 1 Affinity Chromatography: History, Perspectives, Limitations and Prospects Ana Cecí­lia A. Roque and Christopher R. Lowe VARIOUS MODES OF AFFINITY CHROMATOGRAPHY 2 Immobilized Metal Ion Affinity Chromatography of Native Proteins Adam Charlton and Michael Zachariou 3 Affinity Precipitation of Proteins Using Metal Chelates Ashok Kumar, Igor Yu. Galaev and Bo Mattiasson 4 Immunoaffinity Chromatography Stuart R. Gallant, Vish Koppaka and Nick Zecherle 5 Dye-ligand Chromatography Stuart R. Gallant, Vish Koppaka and Nick Zecherle 6 Purification of Proteins Using Displacement Chromatography Nihal Tugcu AFFINITY CHROMATOGRAPHY USING PURIFICATION TAGS 7 Rationally Designed Ligands for use in Affinity Chromatography: an Artificial Protein L Ana Cecí­lia A. Roque and Christopher Lowe 8 Phage display of Peptides in Ligand Selection for use in Affinity Chromatography Joanne L Casey, Andrew M. Coley and Michael Foley 9 Preparation, Analysis and Use of an Affinity Adsorbent for the Purification of GST Fusion Protein Gareth M. Forde 10 Immobilized Metal Ion Affinity Chromatography of Histidine-tagged fusion proteins Adam Charlton and Michael Zachariou 11 Methods for the Purification of HQ-Tagged Proteins Beckky Godat, Laurie Engel, Natalie Betz and Tonny M. Johnson 12 Amylose Affinity Chromatography of Maltose-Binding Protein: Purification by both Native and Novel Matrix-Assisted Dialysis-Refolding Methods. Leonard K. Pattenden and Walter G. Thomas 13 Methods for the Detection of Protein-Protein and Protein-DNA Interactions using Halo-Tag(TM) Marjeta Urh, Danette Hartzell, Jacqui Mendez and Keith Wood 14 Site-specific Cleavage of Fusion Proteins Adam Charlton 15 The Use of TAGzyme for the efficient Removal of N-terminal His-tags José Arnau, Conni Lauritzen, Gitte E. Petersen and John Pedersen VARIOUS APPLICATIONS OF AFFINITY CHROMATOGRAPHY. 16 Affinity Processing of Cell-containing Feeds Using Monolithic Macroporous Hydrogels, Cryogels Igor Yu. Galaev and Bo Mattiasson 17 Monolithic Bioreactors for Macromolecules Mojca Ben_ina, Katja Ben_ina, Ale_ Podgornik and Ale_ _trancar 18 Plasmid DNA Purification via the Use of a Dual Affinity Protein Gareth M. Forde 19 Affinity Chromatography of Phosphorylated Proteins Grigoriy S. Tchaga 20 Protein Separation Using Phospholipid Chromatography Tzong-Hsien Lee and Marie-Isabel Aguilar 21 Analysis of Proteins in Solution Using Affinity Capillary Electrophoresis Neils H.H. Heegaard, Christian Schou and Jesper Østergaard

Kritikerstimmen

From the reviews of the second edition: "The book consists of three parts including 21 chapters that have been written by a large group of authors from many countries. ... recommends this book as a manual for many specialists involved in purification of biomolecules in various fields of biochemistry, molecular biology, biotechnology, and bioorganic chemistry. The book can also be recommended as a handbook for university students and their teachers as well as for other schools preparing specialists in the above-mentioned fields." (G. Ya. Wiederschain, Biochemistry, November, 2008)


ISBN 1-617377-01-5, ISBN 1-61-737701-5, ISBN 1-61737-701-5, ISBN 1-617-37701-5, ISBN 1-6173-7701-5

ISBN 978-1-617377-01-3, ISBN 978-1-61-737701-3, ISBN 978-1-61737-701-3, ISBN 978-1-617-37701-3, ISBN 978-1-6173-7701-3

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